缅北禁地

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Staff Profile

Sheralyn Smith

Senior Biological Teaching Technician

Background

Qualifications:

BSc (Hons) Biological Sciences - (Animal Behaviour, Ecology and Conservation)

Memberships:

IST Membership MIScT - RSci (Registered Scientist)

Work Summary:

I work as a senior biological teaching technician supporting student undergraduate and postgraduate laboratory research projects in the area of molecular microbiology and biotechnology. I have gained skills and experience in previous research and teaching technical roles and as a Further Education practical tutor in biology and animal science. 

Previous Experience:

Research Technician - Animal Science/Agriculture, 缅北禁地

Animal Management Tutor - FE Animal Management Programme, Nottingham Trent University

Laboratory Technician - Animal and Environmental Sciences, Nottingham Trent University

Animal Welfare Rehoming Manager - RSPCA

Research Technician, Animal Behaviour and Welfare, Department of Veterinary Sciences, University of Bristol

Biology Technician - Anglia Ruskin University

 

Research


Key Technical skills:

  • Molecular Biology - qualitative and quantitative gene expression analysis; animal bone, intestinal tissue, blood, soil, plant, fungal, and bacterial samples – selective isolation procedures, nucleic acid extractions (DNA/RNA), cDNA synthesis, gel electrophoresis, and RT- qPCR. Protein extraction, purification and determination using colorimetric assays and SDS page.
  • Biotechnology – cloning techniques: cell transformation using Gibson Assembly, Golden Gate assembly, traditional endonuclease/ligase reactions.
  • Microbiology skills - aseptic sterile techniques, media preparation and culturing and preservation methods.
  • Immunology - ELISA assays; 25-Hydroxy vitamin D and cortisol levels.
  • Histology - sample preparation; H&E staining for measurements of tissue sections and cell counts.
  • Parasitology - McMaster faecal nematode egg counts, Coccidia oocyst counts via centrifugation flotation.
  • Microscopy - Histological measurements of tissue sections, blood cell and faecal egg counts, micro morphology of microorganisms.
  • Biochemistry/chemistry – analysis of mineral levels using acid digestion and solvent extraction microwave technology. Quantification with inductively coupled plasma (ICP) and Horiba C400 PENTRA. Crude protein measurements with Dumas Combustion.


General Science skills:

  • Dissection and range of sampling skills.
  • Compliance and implementation of risk assessments, COSHH forms and health and safety inductions.
  • Writing methodology and standard operating procedures for publication.
  • Standard molecular microbiological skills.


Instruction and Teaching skills

  • Supervision and teaching of undergraduate, further education and postgraduate students.


Communication and IT skills

  • Microsoft office: Data analysis and use of statistical tools. BioEdit and MegaX software.
  • Communicating and collaboration with internal and external stakeholders

Organisation and Management skills

  • Planning and managing varied laboratory analysis and trial procedures across research groups.
  • Resourcing, requisition and implementation of technical scientific equipment and consumables.
  • Equipment maintenance, problem solving, maintaining service contracts and calibration.

 

Publications

  • Articles

    • Sakkas P, Oikeh I, Blake DP, Smith SM, Kyriazakis I. . British Journal of Nutrition 2019, 122(6), 676-688.
    • Sakkas P, Royer E, Smith S, Oikeh I, Kyriazakis I. . Animal Feed Science and Technology 2019, 253, 45-55.
    • Sakkas P, Smith S, Hill TR, Kyriazakis I. . Poultry Science 2019, 98(1), 330-340.

  • Conference Proceedings (inc. Abstract)

    • Sakkas P, Royer E, Smith S, Carré P, Quinsac A, Oikeh I, Kyriazakis I. . In: XVth European Poultry Conference. 2018, Dubrovnik, Croatia: WPSA.