缅北禁地

Research Interests

My current role within the i-sense EPSRC IRC in Early Warning Sensing Systems for Infectious Diseases involves the use of isothermal amplification platforms for the rapid detection of infectious organisms such as C. difficile and MRSA as well as the development of capture reagents against novel bacterial biomarkers. Earlier Postdoctoral positions involved investigating the translocation of toxins produced by E. coli, through the Gram negative outer membrane with a view to use these molecules as novel antibiotics. A biophysics based approach was used to study the mechanism of toxin translocation through the bacterial membrane, spanning techniques including small angle neutron scattering/X-ray scattering, circular dichroism, surface plasmon resonance and isothermal titration calorimetry. 

Current and Recent Funding 

Researcher Co-investigator:   i-sense2, IRC in Agile Early Warning Sensing Systems for Infectious Diseases and Antimicrobial Resistance: EPSRC IRC (£3.9M, October 2018 -September 2022).

Postdoctoral Researcher:   Ultra-Sensitive Enhanced NanoSensing of Anti-Microbial Resistance (u-Sense): EPSRC IRC Next Steps Plus award (£1.39M October 2018 - August 2022).

Postdoctoral Researcher: Early-Warning Sensing Systems for Infectious Diseases: EPSRC IRC (£11.1M, Oct 2013 - Sept 2019) 

• Articles

  • Hassanain WA, Spoors J, Johnson CL, Faulds K, Keegan N, Graham D. . Analyst 2021, 146(14), 4495-4505.
  • Huang Y, Soliakov A, Le Brun AP, Macdonald C, Johnson CL, Solovyova AS, Waller H, Moore GR, Lakey JH. . Biochemistry 2019, 58(48), 4882-4892.
  • Lawry BM, Johnson CL, Flanagan K, Spoors JA, McNeil CJ, Wipat A, Keegan N. . Analytical Chemistry 2018, 90(22), 13475-13482.
  • Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH. . Biophysical Journal 2017, 113(8), 1673-1684.
  • Johnson CL, Ridley H, Marchetti R, Silipo A, Griffin DC, Crawford L, Bonev B, Molinaro A, Lakey JH. . Molecular Microbiology 2014, 92(3), 440-452.
  • Johnson CL, Ridley H, Pengelly RJ, Salleh MZ, Lakey JH. . Molecular Microbiology 2013, 89(1), 84-95.
  • Clifton LA, Johnson CL, Solovyova AS, Callow P, Weiss KL, Ridley H, Le Brun AP, Kinane CJ, Webster JRP, Holt SA, Lakey JH. . Journal of Biological Chemistry 2012, 287(1), 337-346.
  • Zhao XA, Hume SL, Johnson C, Thompson P, Huang JY, Gray J, Lamb HK, Hawkins AR. . Protein Science 2010, 19(7), 1405-1419.
  • Kotaka M, Johnson C, Lamb HK, Hawkins AR, Ren J, Stammers DK. . Journal of Molecular Biology 2008, 381(2), 373-382.
  • Nichols CE, Johnson C, Lockyer M, Charles IG, Lamb HK, Hawkins AR, Stammers DK. . Proteins: Structure, Function and Genetics 2006, 64(1), 111-123.
  • Lamb HK, Ren J, Park A, Johnson C, Leslie K, Cocklin S, Thompson P, Mee C, Cooper A, Stammers DK, Hawkins AR. . Protein Science 2004, 13(12), 3127-3138.
  • Lamb HK, Leslie K, Dodds AL, Nutley M, Cooper A, Johnson C, Thompson P, Stammers DK, Hawkins AR. . Journal of Biological Chemistry 2003, 278(34), 32107-32114.

• Book Chapter

  • Ridley H, Johnson CL, Lakey JH. . In: Proteins: Membrane Binding and Pore Formation. Berlin: Springer, 2010, pp.81-90.

• Conference Proceedings (inc. Abstracts)

  • Arunmanee W, Solovyova A, Johnson CL, Heenan RK, Lakey JH. . In: 58th Annual Meeting of the Biophysical-Society. 2014, San Francisco, CA: Cell Press.
  • Johnson CL, Pengelly RJ, Ridley H, Lakey JH. . In: 56th Annual Meeting of the Biophysical Society. 2012, San Diego, CA: Cell Press.
  • Johnson CL, Clifton LA, Solovyova A, Callow P, Weiss KL, Ridley H, Le Brun AP, Holt SA, Lakey JH. . In: 55th Annual Meeting of the Biophysical Society. 2011, Baltimore, MD: Cell Press.

• Review

  • Hassanain WA, Johnson CL, Faulds K, Graham D, Keegan N. . Analyst 2022, 21(147), 4674-4700.