缅北禁地

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Staff Profile

Professor David Lydall

Professor of Genome Stability

Background

Qualifications

BSc Environmental Sciences University of Warwick 1984
PhD University of London 1988

Memberships

Genetics Society

Honours and Awards

Long Eaton Junior Angling Champion 1977
EMBO Long Term Fellowship 1988-1990

Research

Research Interests

Cellular responses to damaged telomeres in budding yeast

Eukaryotic cells use a variety of mechanisms to protect themselves from the harmful effects of DNA damage. DNA repair enzymes recognise and remove damage, checkpoint control pathways delay cell division while repair occurs, and in some cases cell death or apoptosis is activated to ensure that damaged cells are removed from organisms. Human genetic defects in DNA-damage responses lead to diseases associated with ageing and cancer. For example, Werner's syndrome, a disease associated with premature human ageing, is associated with a mutation in a gene encoding a DNA repair protein. In cancer, more than half of all human tumours contain mutations in the p53 checkpoint protein.

Our lab uses genetic, molecular and biochemical approaches to understand the interplay between DNA damage responses and telomeres in the model organism Saccharomyces cerevisiae (budding yeast). The telomere is a special DNA-protein complex at the end of eukaryotic chromosomes that caps the ends of chromosomes to stop them being recognised by DNA repair and checkpoint complexes. By conditionally inactivating telomere capping proteins in yeast we can monitor the effects of DNA repair and checkpoint genes at telomeres. This permits us to generate models for how DNA damage checkpoint pathways are stimulated by uncapped telomeres and by other types of damage. Since the biochemical mechanisms underlying DNA-damage responses are largely conserved between yeast and human cells our studies in a simple, yet powerful model system, are of direct relevance to human health.

Publications
  • Rodrigues J, Lydall D. . Current Genetics 2018, 64(5), 1105-1116.
  • Markiewicz-Potoczny M, Lisby M, Lydall D. . Genetics 2018, 209(1), 129-141.
  • Holstein E-M, Lawless C, Banks P, Lydall D. . In: Marco Muzi-Falconi, Grant W Brown, ed. Genome Instability: Methods and Protocols. New York: Humana Press Inc, 2018, pp.575-597.
  • Torrance V, Lydall D. . PLoS Genetics 2018, 14(8), e1007523.
  • Rodrigues J, Lydall D. . Nucleic Acids Research 2018, 46(2), 621-634.
  • Lie S, Banks P, Lawless C, Lydall D, Petersen J. . Open Biology 2018, 8(5), 180015.
  • Rodrigues J, Banks P, Lydall D. . G3: Genes, Genomes, Genetics 2018, 8(5), 1807-1816.
  • Makrantoni V, Ciesiolka A, Lawless C, Fernius J, Marston A, Lydall D, Stark MJR. . G3: Genes, Genomes, Genetics 2017, 7(9), 3203-3215.
  • Holstein EM, Ngo G, Lawless C, Banks P, Greetham M, Wilkinson D, Lydall D. . G3: Genes, Genomes, Genetics 2017, 7(7), 2375-2389.
  • Heydari JJ, Lawless C, Lydall DA, Wilkinson DJ. . Journal of the Royal Statistics Society: Series C (Applied Statistics) 2016, 65(3), 367-393.
  • Markiewicz-Potoczny M, Lydall D. . Cell Cycle 2016, 15(20), 2732-2741.
  • Ikeh MA, Kastora SL, Day AM, Herrero-de-Dios CM, Tarrant E, Waldron KJ, Banks AP, Bain JM, Lydall D, Veal EA, MacCallum DM, Erwig LP, Brown AJ, Quinn J. . Molecular Biology of the Cell 2016, 27(17), 2784-2801.
  • Xue Y, Marvin ME, Ivanova IG, Lydall D, Louis EJ, Maringele L. . Aging Cell 2016, 15(3), 553-562.
  • Dubarry M, Lawless C, Banks AP, Cockell S, Lydall D. . G3: Genes, Genomes, Genetics 2015, 5(10), 2187-2197.
  • Narayanan S, Dubarry M, Lawless C, Banks AP, Wilkinson DJ, Whitehall SK, Lydall D. . PloS ONE 2015, 10(7), e0132240.
  • Ngo GHP, Lydall D. . Nucleic Acids Research 2015, 43(10), 5017-5032.
  • Greetham M, Skordalakes E, Lydall D, Connolly BA. . Journal of Molecular Biology 2015, 427(19), 3023-3030.
  • Heydari J, Lawless C, Lydall DA, Wilkinson DJ. . Biosystems 2014, 122, 55-72.
  • Holstein E-M, Clark KRM, Lydall DA. . Cell Reports 2014, 7(4), 1259-1269.
  • Ngo GHP, Balakrishnan L, Dubarry M, Campbell JL, Lydall D. . Nucleic Acids Research 2014, 42(16), 10516-10528.
  • Andrew EJ, Merchan S, Lawless C, Banks AP, Wilkinson DJ, Lydall D. . PLoS One 2013, 8(6), e66242.
  • Dong K, Addinall S, Lydall D, Rutherford JC. . Molecular and Cellular Biology 2013, 33(20), 4041-4050.
  • Banks AP, Lawless C, Lydall DA. . Journal of Visualised Experiments 2012, 66, e4018.
  • Dewar JM, Lydall D. . Chromosoma 2012, 121(2), 117-130.
  • Weile J, Pocock M, Cockell SJ, Lord P, Dewar JM, Holstein EM, Wilkinson D, Lydall D, Hallinan J, Wipat A. . Bioinformatics 2011, 27(9), 1299-1306.
  • Chang H, Lawless C, Addinall SG, Oexle S, Taschuk M, Wipat A, Wilkinson DJ, Lydall D. . G3: Genes, Genomes, Genetics 2011, 1(3), 197-208.
  • Addinall SG, Holstein EM, Lawless C, Yu M, Chapman K, Banks AP, Ngo HP, Maringele L, Taschuk M, Young A, Ciesiolka A, Lister AL, Wipat A, Wilkinson DJ, Lydall D. . PLoS Genetics 2011, 7(4), e1001362.
  • Lawless C, Wilkinson DJ, Young A, Addinall SG, Lydall DA. . BMC Bioinformatics 2010, 11(1), 287.
  • Dewar JM, Lydall D. . EMBO Journal 2010, 29(23), 4020-4034.
  • Ngo HP, Lydall D. . PLoS Genetics 2010, 6(8), e1001072.
  • Dewar JM, Lydall D. . Molecular Cell 2010, 38(6), 777-779.
  • Lydall D. . EMBO Journal 2009, 28(15), 2174-2187.
  • LeBel C, Rosonina E, Sealey DCF, Pryde F, Lydall D, Maringele L, Harrington LA. . Genetics 2009, 182(3), 671-684.
  • Greenall A, Lei GY, Swan DC, James K, Wang LM, Peters H, Wipat A, Wilkinson DJ, Lydall D. . Genome Biology 2008, 9(10), R146.
  • Addinall SG, Downey M, Yu M, Zubko MK, Dewar J, Leake A, Hallinan J, Shaw O, James K, Wilkinson DJ, Wipat A, Durocher D, Lydall DA. . Genetics 2008, 180(4), 2251-2266.
  • Morin I, Ngo H-P, Greenall A, Zubko MK, Morrice N, Lydall D. . EMBO Journal 2008, 27(18), 2400-2410.
  • Lazzaro F, Sapountzi V, Granata M, Pellicioli A, Vaze M, Haber JE, Plevani P, Lydall D, Muzi-Falconi M. . EMBO Journal 2008, 27(10), 1502-1512.
  • Proctor CJ, Lydall D, Boys RJ, Gillespie CS, Shanley D, Wilkinson DJ, Kirkwood TBL. . Journal of the Royal Society Interface 2007, 4(12), 73-90.
  • Tsolou A, Lydall D. . DNA Repair 2007, 6(11), 1607-1617.
  • Downey M, Houlsworth R, Maringele L, Rollie A, Brehme M, Galicia S, Guillard S, Partington M, Zubko MK, Krogan NJ, Emili A, Greenblatt JF, Harrington L, Lydall D, Durocher D. . Cell 2006, 124(6), 1155-1168.
  • Zubko MK, Maringele L, Foster SS, Lydall D. . Methods in Enzymology 2006, 409, 285-300.
  • Zubko MK, Lydall D. . Nature Cell Biology 2006, 8(7), 734-740.
  • Foster SS, Zubko MK, Guillard S, Lydall D. . DNA Repair 2006, 5(7), 840-851.
  • Lydall DA, Whitehall SK. . DNA Repair 2005, 4(10), 1195-1207.
  • Maringele L, Lydall D. . In: Xiao, W, ed. Yeast Protocols. Totawa, New Jersey: Humana Press, 2005, pp.65-74.
  • Maringele L, Lydall D. . Cell Cycle 2005, 4(6), 747-751.
  • Blankley RT, Lydall D. . Journal of Cell Science 2004, 117(4), 601-608.
  • Zubko MK, Guillard S, Lydall D. . Genetics 2004, 168(1), 103-115.
  • Maringele L, Lydall D. . Genetics 2004, 166(4), 1641-1649.
  • Jia X, Weinert T, Lydall D. . Genetics 2004, 166(2), 753-764.
  • Maringele L, Lydall D. . Genes and Development 2004, 18(21), 2663-2675.
  • Lydall D. . Journal of Cell Science 2003, 116(20), 4057-4065.
  • Maringele L, Lydall D. . Genes & Development 2002, 16(15), 1919-1933.
  • Booth C, Griffith E, Brady G, Lydall D. . Nucleic Acids Research 2001, 29(21), 4414-4422.
  • Lydall D. . In: W.L. Fangman;T. Kishimoto;M. Kohiyama;C. Coath, ed. Cell Division and the Replicon: From workshop IX, The Human Frontiers Science Program based on a meeting held in Strasbourg from May 31 to June 2 1999. Strasbourg, France: Human Frontier Science Program, 2000.
  • Loy CJ, Lydall D, Surana U. . Journal of Molecular Cell Biology 1999, 19(5), 3312-3327.
  • Weinert T, Lydall D. Puzzles and pathways in DNA damage checkpoints in yeast. In: J.A. Nickoloff;M. Hoekstra, ed. DNA damage and repair ; Volume 1: DNA repair in prokaryotes and Lower Eukaryotes. Totawa New Jersey, USA: Humana Press, 1998.
  • Lydall D, Weinert T. G2/M checkpoint genes of Saccharomyces cerevisiae: further evidence for roles in DNA replication and/or repair. Molecular and General Genetics 1997, 256(6), 638-51.
  • Lydall D, Weinert T. Use of cdc13-1-induced DNA damage to study effects of checkpoint genes on DNA damage processing. Methods in Enzymology 1997, 283, 410-24.
  • Lydall D, Nikolsky Y, Bishop DK, Weinert T. A meiotic recombination checkpoint controlled by mitotic checkpoint genes. Nature 1996, 383(6603), 840-3.
  • Lydall D, Weinert T. From DNA damage to cell cycle arrest and suicide: a budding yeast perspective. Current Opinion in Genetics and Development 1996, 6(1), 4-11.
  • Lydall D, Weinert T. Yeast checkpoint genes in DNA damage processing: implications for repair and arrest. Science 1995, 270(5241), 1488-91.
  • Weinert T, Lydall D. Cell cycle checkpoints, genetic instability and cancer. Seminars in Cancer Biology 1993, 4(2), 129-40.
  • Lydall D, Ammerer G, Nasmyth K. A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription. Genes and Development 1991, 5(12B), 2405-19.
  • Taba MR, Muroff I, Lydall D, Tebb G, Nasmyth K. Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast. Genes and Development 1991, 5(11), 2000-13.
  • Knox RJ, Lydall DA, Friedlos F, Basham C, Rawlings CJ, Roberts JJ. The Walker 256 carcinoma: a cell type inherently sensitive only to those difunctional agents that can form DNA interstrand crosslinks. Mutation Research 1991, 255(3), 227-40.
  • Nasmyth K, Adolf G, Lydall D, Seddon A. The identification of a second cell cycle control on the HO promoter in yeast: cell cycle regulation of SW15 nuclear entry. Cell 1990, 62(4), 631-47.
  • Knox RJ, Lydall DA, Friedlos F, Basham C, Roberts JJ. The effect of monofunctional or difunctional platinum adducts and of various other associated DNA damage on the expression of transfected DNA in mammalian cell lines sensitive or resistant to difunctional agents. Biochimica et Biophysica Acta 1987, 908(3), 214-23.
  • Roberts JJ, Pera MF, Knox RJ, Friedlos F, Lydall DA. The role of platinum drug-DNA interactions in cellular toxicity and antitumour effects. In: M. Nicolini, ed. Fifth International Symposium on Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy. Boston, USA. Abano, Padua, Italy: Martinus Nijhoff Publishing, 1987, pp.16-31.
  • Roberts JJ, Knox RJ, Friedlos F, Lydall DA. DNA as the target for the cytotoxic and antitumour action of platinum co-ordination complexes: comparative in vitro and in vivo studies of cisplatin and carboplatin. In: D. C. H. McBrien;T. F. Slater, ed. Biochemical Mechanisms of Platinum Antitumour Drugs. Oxford, England: IRL Press, 1986, pp.29-64.
  • Knox RJ, Friedlos F, Lydall DA, Roberts JJ. Mechanism of cytotoxicity of anticancer platinum drugs: evidence that cis-diamminedichloroplatinum(II) and cis-diammine-(1,1-cyclobutanedicarboxylato)platinum(II) differ only in the kinetics of their interaction with DNA. Cancer Research 1986, 46(4 Pt 2), 1972-9.