缅北禁地

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Staff Profile

Dr Thomas Nicholls

Research Fellow

  • Telephone: +44 (0) 191 208 6291
  • Address: Biosciences Institute
    The Medical School
    缅北禁地
    Framlington Place
    缅北禁地 upon Tyne
    NE2 4HH
Background

Research

Sir Henry Dale Fellow (2019-2026), Wellcome Centre for Mitochondrial Research, 缅北禁地.

Rosetrees and Stoneygate Trust Research Fellow (2018-2025), Wellcome Centre for Mitochondrial Research, 缅北禁地.

Research associate, University of Gothenburg (2014-2018). Lab of Prof. Claes Gustafsson.

Investigator Scientist, MRC Mitochondrial Biology Unit, Cambridge (2013-2014), lab of Dr Michal Minczuk.

Education

PhD Mitochondrial Genetics, University of Cambridge (2009-2013), lab of Dr Michal Minczuk.

BSc (Hons.) Molecular Biology and Biochemistry, University of Durham (2006-2009)



Research

Mitochondrial DNA maintenance, propagation and disease

Human cells contain several thousand copies of the mitochondrial genome (mtDNA) which are packaged into nucleoprotein complexes termed nucleoids. These genomes are replicated throughout the cell cycle, and are found evenly distributed around the dynamic mitochondrial network. At the end of mtDNA replication the genomes must be disentangled and resolved, before then being distributed within the cell. Our work uses molecular, biochemical and cell biology techniques to study these processes in human cells. The aims of this work are: (1) to determine how mtDNA is disentangled following DNA replication; (2) to identify and characterise factors that are required for mtDNA resolution and segregation; and (3) to elucidate the molecular basis of human mitochondrial diseases linked to impaired mtDNA resolution and segregation.


Research Team

Katja Menger - Research Associate

Christin Albus - Research Associate

Ashley Ngan - PhD Student



Teaching

BGM3060 - Diagnostic Medical Genetics - Lecturer

CMB3000 - Project Supervisor

MMB8030 - Genetic Medicine - Lecturer

MMB8034 - Mitochondrial Biology and Medicine - Module co-lead and lecturer

MRes Induction Week - Lecturer

BMS undergraduate tutor

MRes tutor


Publications

  • Articles

    • Bernardino Gomes TM, Vincent AE, Menger KE, Stewart JB, Nicholls TJ. . Biochemical Journal 2024, 481(11), 683-715.
    • Erdinc D, Rodriguez-Luis A, Fassad MR, Mackenzie S, Watson CM, Valenzuela S, Xie X, Menger KE, Sergeant K, Craig K, Hopton S, Falkous G, Poulton J, Garcia-Moreno H, Giunti P, de Moura Aschoff CA, Morales Saute JA, Kirby AJ, Toro C, Wolfe L, Novacic D, Greenbaum L, Eliyahu A, Barel O, Anikster Y, McFarland R, Gorman GS, Schaefer AM, Gustafsson CM, Taylor RW, Falkenberg M, Nicholls TJ. . EMBO Molecular Medicine 2023, 15(5), e16775.
    • Menger KE, Chapman J, Díaz-Maldonado H, Khazeem MM, Deen D, Erdinc D, Casement JW, Di Leo V, Pyle A, Rodríguez-Luis A, Cowell IG, Falkenberg M, Austin CA, Nicholls TJ. . Nucleic Acids Research 2022, gkac857.
    • Tan BG, Mutti CD, Shi Y, Xie X, Zhu X, Silva-Pinheiro P, Menger KE, Díaz-Maldonado H, Wei W, Nicholls TJ, Chinnery PF, Minczuk M, Falkenberg M, Gustafsson CM. . Molecular Cell 2022, 82(19), 3646-3660.e9.
    • Misic J, Milenkovic D, Al-Behadili A, Xie X, Jiang M, Jiang S, Filograna R, Koolmeister C, Siira SJ, Jenninger L, Filipovska A, Clausen AR, Caporali L, Valentino ML, La Morgia C, Carelli V, Nicholls TJ, Wredenberg A, Falkenberg M, Larsson NG. . Nucleic Acids Research 2022, 50(15), 8749–8766.
    • Jiang M, Xie X, Zhu X, Jiang S, Milenkovic D, Misic J, Shi Y, Tandukar N, Li X, Atanassov I, Jenninger L, Hoberg E, Albarran-Gutierrez S, Szilagyi Z, Macao B, Siira SJ, Carelli V, Griffith JD, Gustafsson CM, Nicholls TJ, Filipovska A, Larsson NG, Falkenberg M. . Science Advances 2021, 7(27), eabf8631.
    • Strakova A, Nicholls TJ, Baez-Ortega A, Ní Leathlobhair M, Sampson AT, Hughes K, Bolton IAG, Gori K, Wang J, Airikkala-Otter I, Allen JL, Allum KM, Arnold CL, Bansse-Issa L, Bhutia TN, Bisson JL, Blank K, Briceño C, Castillo Domracheva A, Corrigan AM, Cran HR, Crawford JT, Cutter SM, Davis E, de Castro KF, De Nardi AB, de Vos AP, Keenan LD, Donelan EM, Espinoza Huerta AR, Faramade IA, Fazil A, Fotopoulou E, Fruean SN, Gallardo-Arrieta F, Glebova O, Gouletsou PG, Häfelin Manrique RF, Henriques JJGP, Horta RS, Ignatenko N, Kane Y, King C, Koenig D, Krupa A, Kruzeniski SJ, Lanza-Perea M, Lazyan M, Lopez Quintana AM, Losfelt T, Marino G, Martínez Castañeda S, Martínez-López MF, Masuruli BM, Meyer M, Migneco EJ, Nakanwagi B, Neal KB, Neunzig W, Nixon SJ, Ortega-Pacheco A, Pedraza-Ordoñez F, Peleteiro MC, Polak K, Pye RJ, Ramirez-Ante JC, Reece JF, Rojas Gutierrez J, Sadia H, Schmeling SK, Shamanova O, Sherlock AG, Steenland-Smit AE, Svitich A, Tapia Martínez LJ, Ngoka IT, Torres CG, Tudor EM, van der Wel MG, Vitalaru BA, Vural SA, Walkinton O, Wehrle-Martinez AS, Widdowson SAE, Zvarich I, Chinnery PF, Falkenberg M, Gustafsson CM, Murchison EP. . Nature Communications 2020, 11, 3059.
    • Lehmann D, Tuppen HAL, Campbell G, Alston CL, Lawless C, Rosa HS, Rocha MC, Reeve AK, Nicholls TJ, Deschauer M, Zierz S, Taylor RW, Turnbull DM, Vincent AE. . Nucleic Acids Research 2019, 47(14), 7430–7443.
    • Andreazza S, Samstag CL, Sanchez-Martinez A, Fernandez-Vizarra E, Gomez-Duran A, Lee JJ, Tufi R, Hipp MJ, Schmidt EK, Nicholls TJ, Gammage PA, Chinnery PF, Minczuk M, Pallanck LJ, Kennedy SR, Whitworth AJ. . Nature Communications 2019, 10, 3280.
    • Nicholls TJ, Spåhr H, Jiang S, Siira SJ, Koolmeister C, Sharma S, Kauppila JHK, Jiang M, Kaever V, Rackham O, Chabes A, Falkenberg M, Filipovska A, Larsson NG, Gustafsson CM. . Molecular Cell 2019, 76, 1-13.
    • Nicholls TJ, Nadalutti CA, Motori E, Sommerville EW, Gorman GS, Basu S, Hoberg E, Turnbull DM, Chinnery PF, Larsson N-G, Larsson E, Falkenberg M, Taylor RW, Griffith JD, Gustafsson CM. . Molecular Cell 2018, 69(1), 9-23.e6.
    • Matic S, Jiang M, Nicholls TJ, Uhler JP, Dirksen-Schwanenland C, Polosa PL, Simard ML, Li X, Atanassov I, Rackham O, Filipovska A, Stewart JB, Falkenberg M, Larsson NG, Milenkovic D. . Nature Communications 2018, 9(1), 1202.
    • Uhler JP, Thörn C, Nicholls TJ, Matic S, Milenkovic D, Gustafsson CM, Falkenberg M. . Nucleic Acids Research 2016, 44(12), 5861-5871.
    • Kopajtich R, Nicholls TJ, Rorbach J, Metodiev MD, Freisinger P, Mandel H, Vanlander A, Ghezzi D, Carrozzo R, Taylor RW, Marquard K, Murayama K, Wieland T, Schwarzmayr T, Mayr JA, Pearce SF, Powell CA, Saada A, Ohtake A, Invemizzi F, Lamantea E, Sommerville EW, Pyle A, Chinnery PF, Crushell E, Okazaki Y, Kohda M, Kishita Y, Tokuzawa Y, Assouline Z, Rio M, Feillet F, de Camaret BM, Chretien D, Munnich A, Menten B, Sante T, Smet J, Regal L, Lorber A, Khoury A, Zeviani M, Strom TM, Meitinger T, Bertini ES, Van Coster R, Klopstock T, Rotig A, Haack TB, Minczuk M, Prokisch H. . American Journal of Human Genetics 2014, 95(6), 708-720.
    • Rorbach J, Boesch P, Gammage PA, Nicholls TJ, Pearce SF, Patel D, Hauser A, Perocchi F, Minczuk M. . Molecular Biology of the Cell 2014, 25(17), 2539-2676.
    • Nicholls TJ, Zsurka G, Peeva V, Schöler S, Szczesny RJ, Cysewski D, Reyes A, Kornblum C, Sciacco M, Moggio M, Dziembowski A, Kunz WS, Minczuk M. . Human Molecular Genetics 2014, 23(23), 6147-6162.
    • Kornblum C, Nicholls TJ, Haack TB, Schöler S, Peeva V, Danhauser K, Hallmann K, Zsurka G, Rorbach J, Iuso A, Wieland T, Sciacco M, Ronchi D, Comi GP, Moggio M, Quinzii CM, DiMauro S, Calvo SE, Mootha VK, Klopstock T, Strom TM, Meitinger T, Minczuk M, Kunz WS, Prokisch H. Loss-of-function mutations in MGME1 impair mtDNA replication and cause multisystemic mitochondrial disease. Nature Genetics 2013, 45, 214–219.
    • Haack TB, Kopajtich R, Freisinger P, Wieland T, Rorbach J, Nicholls TJ, Baruffini E, Walther A, Danhauser K, Zimmermann FA, Husain RA, Schum J, Mundy H, Ferrero I, Strom TM, Meitinger T, Taylor RW, Minczuk M, Mayr JA, Prokisch H. . American Journal of Human Genetics 2013, 93(2), 211-223.
    • Rorbach J, Nicholls TJ, Minczuk M. PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria. Nucleic Acids Research 2011, 39(17), 7750–7763.

  • Reviews

    • Menger KE, Rodriguez-Luis A, Chapman J, Nicholls TJ. . Open Biology 2021, 11(9), 210168.
    • Chapman J, Ng YS, Nicholls TJ. . Life 2020, 10(9), 164.
    • Nicholls TJ, Gustafsson CM. . Trends in Biochemical Sciences 2018, 43(11), 869-881.
    • Powell CA, Nicholls TJ, Minczuk M. . Frontiers in Genetics 2015, 6, 79.
    • Van Haute L, Pearce SF, Powell CA, D'Souza AR, Nicholls TJ, Minczuk M. . Journal of Inherited Metabolic Disease 2015, 38(4), 655-680.
    • Nicholls TJ, Minczuk M. . Experimental Gerontology 2014, 56, 175-181.
    • Nicholls TJ, Rorbach J, Minczuk M. Mitochondria: mitochondrial RNA metabolism and human disease. International Journal of Biochemistry and Cell Biology 2013, 45(4), 845-849.